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The measurement was carried out by demarcating the smallest diameter passing through the center of the muscular fibers Fig. Ten cuts were produced and muscle fibers measured per animal in accordance with the protocol established by Dal Pai The Shapiro-Wilk normality test was performed to verify the distribution of values in the Gaussian curve.
Normality was not verified for the variables corresponding to the muscle diameter and thus, the nonparametric Kruskal-Wallis test with the Dunn post test was used. As for the body mass variable, normality was verified and thus the dependent Student t test was used for the intragroup analysis, in the pre and post-training moments. Muscular strength training protocol. Measurement of the smallest diameter of muscle fibers.
From the analysis of muscle fibers, distinct morphologies were observed in the different groups of animals, both in the analyses obtained from the HE staining and in the NADH-TR reaction Figs. Thus, in general, when observing the HE cuts, an increase in the MF diameter was noted in all groups, independent of the treatment.
In addition, after performing the morphometric analyzes measurements of diameter it was observed that the groups of animals responded differently to treatment Figs. It was verified that when analyzing the fibers in a general manner through the HE staining method, all experimental groups demonstrated hypertrophy of muscle fibers.
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Analysis of body mass in the pre- and post-training moments. Control and GH group. Strength Training Group D: Strength Training and GH group. Staining with hematoxylin and eosin HE of muscle fibers at 10X magnification.
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Strength training and GH group. Arrows indicate muscle fibers. Analysis of fast and slow twitch muscle fibers through the nicotinamide adenine dinucleotide tetrazolium reductase reaction NADH-TR. Diameter of Muscle Fibers through the staining with hematoxylin and eosin HE. Statistical difference when compared to the C group. Diameter of slow twitch muscle fibers through the nicotinamide adenine dinucleotide tetrazolium reductase reaction NADH-TR.
Diameter of fast twitch muscle fibers through the nicotinamide adenine dinucleotide tetrazolium reductase reaction NADH-TR. The measurements were performed from the smallest diameter of the muscle fibers and specific measures of fast FMCR and slow twitch fibers FMCL were also carried out.
It is known that the use of water jumps can recover volume of muscle tissue lost after immobilization Antunes et al. In addition to causing hypertrophy of muscle fibers Moret et al. Differently, the FMCL adapt better to the application of stimuli with less force of contraction and performed for long periods of time, as in the specific case of aerobic training.
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Specificity of physical exercise can increase cellular organelles, such as mitochondria, and thus increase the intramuscular energy substrates and their improved utilization in the aerobic metabolism Castoldi et al.
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Electromyographic activity during one-legged squatting under different foot positions
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