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The Quebrada de Humahuaca is a deep narrow valley or ravine extending along km and presenting two different climates, semi-arid and arid, according to the variation of precipitations [ 31 ]. Mountainous relief influences the total annual precipitations, causing the geographical distribution of rains to be markedly irregular.

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Although rains are torrential and are concentrated from November to March, they do not compensate for the marked water stress present in the area [ 31 ]. In a general sense, NWA is a geographical region defined by the presence of the Andean mountain range, running in a NS direction, as well as several intermountain valleys, quebradas, and a high altitude area more than 3, masl called puna that sustained the development of interconnected societies along its temporal occupation.

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Although NWA has been occupied for more than From about A. Agricultural intensification played a key role in the development of these societies, which cleared vast extensions of land and built agricultural structures e.

While the traditional view of these societies posited that they were organized into chiefdoms that hierarchically ranked settlements levels [ 37 — 39 ], other scholars suggest that communities from the Regional Development Period RDP, ca.

In this sense, regionalization of ceramic styles and material culture in general points to the existence of political fragmentation, which can also be seen in the replacement of previous dispersed hamlets for key nucleated settlements located in defensive locations. This scenario possibly resulted from the presence of interregional conflict, evidenced in the appearance of war related paraphernalia e. Staple goods in communities from NWA temperate valleys included maize Zea mayspotatoes Solanum spp.

Outstandingly, apart from maize, kiwicha A. Although mentioned in the archaeological literature as early as by Ten Kate, it was later studied by different archaeological research teams [ 53 ].

The settlement consists of architectural remains built on the slopes of a hill, a pattern shared with other sites from the Yocavil Valley e. Research conducted by Dr. Williams [ 5455 ] revealed the distribution of the architectural remains in the hill, covering 35 ha, in the following way: Radiocarbon dating allows ascribing the occupation of the site to the years to A. The excavation of one of the residential enclosures located in the base sector Building 6 yielded a number of cultural materials, including metal artifacts and debris, ceramic remains, lithic instruments and by-products, vegetables and faunal remains, and a hearth in the centre of the enclosure.

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The analysis of these materials allowed for the interpretation of this enclosure as a domestic space where quotidian activities were carried out [ 55 ]. Local ceramic styles are abundant in this enclosure i. Many undecorated fragments show fire marks, possibly related to their use as cooking vessels [ 58 ]. The vegetable macroremains found consisted of five different races or variants of maize Colorado, Chaucha, Socorro, Amarillo Chico, and Pisingallo ; several caryopses were burnt, suggesting that they had probably been cooked [ 50 ] Table 2.

Faunal remains found in this enclosure were divided into three components. The first one stratigraphic levels 7 to 10 is mainly composed of camelids Lama sp. The second component stratigraphic levels 3 to 6 presents a variety of resources, including fox Lycalopex sp. In the third component stratigraphic levels 1 and 2 the only osseous remains found were from camelids Lama sp.

In general, faunal remains from this enclosure show the predominant consumption of camelids with a mortality profile of young individuals in two of the three defined components. Additionally, remains of an Andean deer Hippocamelus antisensis, commonly known as taruca were found. Anthropic modifications, like cut marks or thermal alterations, were recorded, suggesting the possible performance of human activities such as slaughter and cooking [ 55 ]. The tomb was located under a big rock which stood on the sides of a ditch forming a kind of natural roof of 2 m wide and 2.

Although the human remains were highly fragmented, osteological analysis allowed determining that at least six individuals were buried, including both adults and subadults [ 60 ].

From this funerary context, five individuals were sampled for isotopic analysis see details in Table 3 using bones that could be clearly associated to an individual.

Carbon and nitrogen results for human bone samples. Ina salvage project was conducted at the site and six tombs were found, along with other archaeological materials [ 61 ]. The tombs were located in what was called Terrace III, and five of them were excavated tombs 1, 2, 3, 4, and 6 Figure 4. The laboratory analysis of the human bone remains allowed establishing the presence of at least 18 individuals, comprising adults of both sexes and unsexed subadults of different ages at death [ 61 ].

Plan of the tombs from Esquina de Huajra. Radiocarbon analyses postulate an occupation span ranging from A. This would imply that the site was occupied and possibly built in a late stage of the Inca Period, continuing this occupation in the initial stage of the Early Colonial Period, although the real control of the territory by the Spaniards was not exerted until A. This is consistent both with the findings of Inca material culture in the tombs and the absence of Spanish objects in the site.

The analyses of the materials recovered suggest the performance of different activities in the sectors of the site. In Terrace I, a possible patio was excavated, which contained a hearth with carbon lenses, ceramic and bone fragments, a stone grinder, red pigment, and several ceramic remains. This occupation floor also contained abundant animal remains, two bone instruments, and a metal instrument [ 6162 ]. The ceramic assemblage contains some of the typical Inca forms present in the provinces of the Empire [ 63 ]: The lithic material obtained points to the performance of reduction activities directed to obtaining base forms [ 61 ].

In turn, Terrace II probably functioned as a circulation path. More than 1, ceramic fragments were found, half of them undecorated and corresponding to medium and large vessels and ollas. A few fragments were from the body of five small vessels, and also bowl fragments were recovered. As far as we know, most of the surface of Terrace III seems to have been used mainly as a funerary space [ 59 ], as six tombs containing the remains of several individuals were found and analyzed.

Five of the six detected tombs could be excavated, and six samples of human bone were collected for isotopic analysis, including adult individuals from both sexes and unsexed subadults from different ages as described in Table 3.

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These bone elements were selected so as to have at least one sample from each tomb. The bone was cleaned with wire brush and washed using ultrasonic bath.

After cleaning, the dried bone was gently crushed into small fragments. The crushed bone was treated with 1 N acetic acid to remove any secondary carbonates at room temperature with periodic evacuation of CO2 for 24 hours. The residue was filtered and rinsed with deionized water to remove any remains of acetic acid.

The carbon dioxide was cryogenically purified and collected for stable isotope ratio analysis. Then the solution was filtered on fiberglass filter, and precipitate was rinsed with deionized water. The precipitate was boiled in deionized water for 6 hours to dissolve collagen and leave humic substances in the precipitate.

The collagen solution was then filtered and dried out to isolate pure collagen. The carbon dioxide and nitrogen were cryogenically separated and purified and collected in the flasks for analyses. At Geochron, a VG Micromass gas source stable isotope ratio mass spectrometer was used.

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No industrial fertilizers were used in order to avoid any potential nitrogen enrichment [ 66 ]. Modern plant samples were corrected for the Suess Effect; that is, the reduction by at least 1. It is, therefore, necessary to correct these samples by adding 1. Only data containing both carbon and nitrogen information were considered, and median values were presented for each type of data i.

The results for analyzed food samples are summarized in Table 4. Carbon results were corrected for postindustrial enrichment. Plot of modern plants from database green markersmodern plants from the current study dark orange markersand archaeological fauna against human bone samples from Esquina de Huajra.

Interestingly, camelid diet from both sites is no far from that of humans, showing only subtle differences. As mentioned above, collagen reflects the protein portion of the diet consumed in the last 5 to 7 years of life. In this regard, the results obtained for both sites show that the ingested proteins came from a C4 source mainly. Moreover, data from carbon apatite suggest that energy also came from a C4 source, as we shall discuss later. Data from this study contrasted to Kellner and Schoeninger [ 25 ] regression lines.

Interestingly, camelid diet is quite similar to that of humans.

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Isotopic data from camelid bone from Esquina de Huajra suggest that their diet was quite homogeneous and that they would correspond to locally raised herds or to animals that lived freely in low altitude grasslands [ 64 ]. In spite of these differences, human diets from both sites seem quite homogeneous, at least for carbon values, as they cluster tightly. This would imply that diets from both adults and subadults and both males and females were quite similar; moreover, they came primarily from a C4 source, as we can see in Figures 4 and 5.

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